Strain Details - ARLG-2829

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Strain Details

Species:	Escherichia coli
Aliases:	MCR1_NJ
Collection:	No
Location Setting:	Tertiary-Care Hospital
Body Site:	Urine
Original Isolation Date:	2014
Outbreak:	No
Biosafety Level:	BSL2
Strain Note:	First colistin and carbapenem resistant strain reported in US. Strain isolated from nephrostomy tube drainage culture. MIC (except colistin) performed using MicroScan Walkaway Plus System (Beckman Coulter). Colistin MIC values determined using Etest strips (bioMerieux).

Demographic Information

Clinical Data:	Yes
Gender:	Male
Region:	New Jersey, United States
Note:	76-year old man with recurrent urinary tract infections and no recent travel history at the time of hospitalization

References

Panels

Strain Characterization

Antimicrobial Profile

MIC Assay
DRUG	MIC (mcg/ml)	Resistance Phenotype	PubMed ID
Amikacin	<=16	Susceptible	27578755
Ampicillin	>16	Resistant	27578755
Ampicillin-Sulbactam	>16/8	Resistant	27578755
Aztreonam	<=8	Susceptible	27578755
Cefazolin	>16	Resistant	27578755
Cefepime	>16	Resistant	27578755
Ceftazidime	>16	Resistant	27578755
Ciprofloxacin	>2	Resistant	27578755
Colistin	3	Resistant	27578755
Ertapenem	>4	Resistant	27578755
Gentamicin	<=4	Susceptible	27578755
Imipenem	>8	Resistant	27578755
Levofloxacin	>4	Resistant	27578755
Meropenem	>8	Resistant	27578755
Nitrofurantoin	<=32	Susceptible	27578755
Piperacillin-Tazobactam	>64	Resistant	27578755
Tigecycline	<=2	Susceptible	27578755
Trimethoprim-Sulfamethoxazole	<=2/38	Susceptible	27578755

Genotypic Profile

Genetic Characterization
Type	Result	PubMed ID
MLST Sequence Type (ST)	Single-locus variant of ST405	27578755

Genetic Characterization
Beta-lactamase bla Genes	Result	PubMed ID
NDM-5	Positive	27578755
OXA-1	Positive	27578755

Genetic Characterization
Gene Resistance Type	Gene	Result	PubMed ID
Aminoglycoside Modifying Enzime Gene	strA	Positive	27578755
Aminoglycoside Modifying Enzime Gene	strB	Positive	27578755
Chloramphenicol Resistance Gene	catB3	Positive	27578755
Chloramphenicol Resistance Gene	floR	Positive	27578755
Colistin Resistance Gene	mcr-1	Positive	27578755
Fluoroquinolone Resistance Gene	aac(6')-Ib-cr	Positive	27578755
Rifampin Resistance Gene	arr-3	Positive	27578755
Sulfonamide Resistance Gene	sul1	Positive	27578755
Sulfonamide Resistance Gene	sul2	Positive	27578755
Tetracycline Resistance Gene	tet(A)	Positive	27578755

Other Genetic Characterization
Additional Characterization	Information Available	PubMed ID
Genome Sequencing	MCR1_NJ draft genome sequence deposited as GenBank accession no. MAJK00000000	27578755
Plasmid Sequencing	Plasmid pMCR1-NJ-IncX4 nucleotide sequence deposited in GenBank with accession no. KX447768	27578755
Plasmid Sequencing	Plasmid pNDM5-NJ-IncX3 nucleotide sequence deposited in GenBank with accession no. KX447767	27578755